Chloroquine calcium phosphate transfection

Discussion in 'Chloroquin' started by autosnab, 13-Mar-2020.

  1. Yakatory Guest

    Chloroquine calcium phosphate transfection


    Although not all of these side effects may occur, if they do occur they may need medical attention. Check with your doctor immediately if any of the following side effects occur while taking chloroquine: Incidence not known Some side effects of chloroquine may occur that usually do not need medical attention.

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    Calcium phosphate transfection is one of the most commonly used methods to deliver plasmid DNA into mammalian cells. Due to its highly consistent pH and salt concentration, our CalPhos calcium phosphate transfection kit gives you the consistent transfection efficiency that you cannot always achieve with home brew versions. The optimized transfection procedure requires that CHO cells to be incubated with calcium phosphate-DNA coprecipitate and chloroquine 100 microM for a total of 16 h. By using high-molecular-weight human genomic DNA as a DNA source for transfection, we show that this procedure is equally efficient for stably transferring a much larger gene, such as the 49-kb human hypoxanthine phosphoribosyltransferase gene. Calcium Phosphate-Mediated Transfection of Adherent Cells. 1. Harvest exponentially growing adherent cells by trypsinization. Resuspend the cells in growth medium containing serum, and centrifuge aliquots containing ∼10 6 cells at 800g 2000 rpm in a Sorvall H1000B rotor for 5 min at 4°C.

    Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects. These side effects may go away during treatment as your body adjusts to the medicine.

    Chloroquine calcium phosphate transfection

    ProFection Mammalian Transfection System - Promega, High-efficiency stable gene transfection using chloroquine.

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  5. Chloroquine phosphate Tablets for Oral Administration, USP. DESCRIPTION. ARALEN, chloroquine phosphate, USP, is a 4-aminoquinoline compound for oral administration. It is a white, odorless, bitter tasting, crystalline substance, freely soluble in water. ARALEN is an antimalarial and amebicidal drug.

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    Chloroquine is an inhibitor of the lysosomal degradation of the DNA which is taken up by the cells, so as leelee said, transfection should have been successful, albeit at a slightly lower level than if you would have added the chloroquine. In my normal transfections, I never add chloroquine and get high efficiencies nevertheless. Calcium phosphate co-precipitation as a transfection method was introduced in the early 1970s Graham and van der Eb, 1973. The method is still popular because the components are easily available and inexpensive, the protocol is easy to use, and it is effective with many different types of cultured cells. Popular chemical-based transfection means such as calcium phosphate co-precipitation are prone to variability; small pH changes can compromise the efficiency of calcium phosphate transfection into the cell and size and quality of the precipitate is critical for successful gene transfer and expression to occur.

     
  6. limp_max New Member

    Please make sure that Javascript and cookies are enabled on your browser and that you are not blocking them from loading. Hydroxychloroquine - LiverTox - NCBI Bookshelf Hydroxychloroquine Availability - 22nd July 2019. Hydroxychloroquine All Licensing, EU CTD Dossiers, Formulation.
     
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